mouse th2 cell line d10 g4 1  (ATCC)

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Quantification of the levels of mRNA encoding WT and SD VPAC2 in D10G4.1 cell transfectants. Each column and bar depicts the mean ± SD of the results of three individual determinations. The corresponding values for mock-transfected D10G4.1 Th2 cells were marginally detectable and were indistinguishable from those of untransfected D10G4.1 Th2 cells. Peaks of mRNA encoding each type of VPAC2 receptor are evident at 24 h and persist without significant change at 48 h. None of the differences between levels of mRNA encoding WT and SD VPAC2 were significant.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques: Transfection

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Western blot of membrane proteins extracted from mouse D10G4.1 Th2 cells transfected 48 h before with plasmids encoding mouse WT and SD VPAC2. The primary reagent for development was mouseanti-FLAG M2 mAb. The first pair of WT-SD lanes received 3 μg of extracted proteins, and the second pair 10 μg of extracted proteins; 10 μg of extract of control (C) untransfected D10G4.1 T cells were loaded into the last lane.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques: Western Blot, Transfection

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Generation of VIP by mouse D10G4.1 Th2 cell transfectants. Each column and bar represents the mean ± SD of results from two studies analyzed in duplicate. None of the differences between levels of VIP generated by WT and SD transfectants was significant.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques: Generated

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Dependence of the level of expression of mRNA encoding IL-4 by mouse D10G4.1 Th2 cell transfectants on endogenous VIP signaling through WT and SD VPAC2. The difference in corrected levels of mRNA encoding IL-4 in stimulated WT and SD VPAC2 transfectants without VIPase is significant with p < 0.05 (+) and that between WT transfectants without and with VIPase is significant with *, p < 0.01; n = 3, paired Student’s t test.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques: Expressing

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Opposite signals from WT and SD VPAC2 to IL-4 secretion by D10G4.1 Th2 cell transfectants stimulated by adherent anti-CD3 plus anti-CD28 mAbs. Control secretion of immunoreactive IL-4 by transfectants in 24 h without VIP was set at 100%. Each column and bar depicts the mean ± SD of the results of three or more studies. The concentrations of VIP between those marked were 3 × 10−9, 3 × 10−8, and 3 × 10−7 M. The significance of differences between IL-4 secretion in the presence and absence of exogenous VIP for each set of transfectants was calculated by a standard paired t test;+, p < 0.05; and *, p < 0.01.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques:

Journal:

Article Title: Differential Signaling of T Cell Generation of IL-4 by Wild-Type and Short-Deletion Variant of Type 2 G Protein-Coupled Receptor for Vasoactive Intestinal Peptide (VPAC 2 ) 1

doi:

Figure Lengend Snippet: Effect of VIP on anti-CD3 stimulated increases in the nuclear level of active Jun B protein in D10G4.1 Th2 cell transfectants. Each column and bar represents the mean ± range of two studies in duplicate. Suspensions of 106 transfectants in 0.6 ml of RPMI 1640 with 10% FBS were preincubated 60 min without or with 10−7 M VIP and then incubated with 1 μg of anti-CD3 Ab for 4 h. Each number in parentheses is the mean increase (+) or decrease (−) in active nuclear Jun B attributable to VIP. The significance of differences in levels of active nuclear Jun B between samples without and with VIP was calculated by a paired t test; *, p < 0.01.

Article Snippet: Cells, nucleofection procedures, and Abs A mouse Th2 cell line designated D10G4.1 was obtained from American Type Culture Collection.

Techniques: Incubation